Full cast of Pee-wee's Playhouse

Paul Reubens

Pee-wee Herman

45 episodes

Lynne Marie Stewart

Miss Yvonne

45 episodes

Laurence Fishburne

Cowboy Curtis

45 episodes

S. Epatha Merkerson

Reba

45 episodes

John Paragon

Jambi

45 episodes

Alison Mork

Chairry (voice) / Magic Screen (voice)

45 episodes

Suzanne Kent

Mrs. Renee

32 episodes

William Marshall

The King of Cartoons

32 episodes

Phil Hartman

Captain Carl

13 episodes

Gilbert Lewis

The King of Cartoons

13 episodes

Shirley Stoler

Mrs. Steve

13 episodes

Gregory Harrison

Conky 2000 (voice) / Knucklehead (voice)

13 episodes

Natasha Lyonne

Opal

13 episodes

Diane Yang

Cher

13 episodes

Alisan Porter

Li'l Punkin

2 episodes

Johann Carlo

Dixie

2 episodes

Shaun Weiss

Elvis

2 episodes

Calvert DeForest

1 episode

Roland Rodriquez

Tito

1 episode

Leslie Jordan

Bubsy

1 episode

Max Robert

Organist

1 episode